The project focuses on mathematical modelling of the overflow metabolism of Escherichia coli that limits both biomass yield and the quality of biotechnologically important products. The regulation of various metabolic pathways in E. coli is studied by experimental and theoretical work. Data from metabolite, mRNA and protein levels are integrated into comprehensive mathematical modelling. Interesting dynamic phenomena such as oscillations will be investigated experimentally and by model simulations.
The quantitative modelling of the regulation of metabolic fluxes around the pyruvate node, in particular the minimization of by-product formation is a prerequisite for optimizing recombinant protein production by E. coli. Due to the close collaboration of the partners with the biopharma-ceutical industry and spin-off companies the resulting knowledge will be efficiently exploited.
Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e.V. - Hans-Knöll-Institut (HKI),
Abteilung Molekulare und Angewandte Mikrobiologie, AG Systembiologie/Bioinformatik
Leitung: PD Dr. Reinhard Guthke
Beutenbergstr. 11a, D-07745 Jena
Tel.: +49-3641- 532 1083, Fax: +49-3641- 532 0803
e-Mail: Reinhard.Guthke
hki-jena.de